The significance of the entrance slit and the diffraction grating in forming a spectral image of the incident light in the image plane is discussed in the articles “An Introduction to a Spectrometer: ...
a, Example of ISM-FLUX on nanorulers consisting of three fluorophores with 40 nm spacing between nearest neighbours. With a single-element detector, none of the nanorulers can be resolved, due to the ...
Omega Optical has recently changed its multispectral fluorescence detection system to flow-cytometry. The hardware was initially produced for confocal applications and consists of pulsed laser ...
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